Difference between revisions of "Part:BBa K2368024"
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| + | <h1>Introduction</h1> | ||
<partinfo>BBa_K2368024 short</partinfo> | <partinfo>BBa_K2368024 short</partinfo> | ||
| + | <p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-6*His tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location. </p> | ||
| + | <p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and His tag. And hope to show the location of T1R3 through immunofluorescence for detecting His tag.</p> | ||
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| − | < | + | <h2>Sequence and Features</h2> |
<partinfo>BBa_K2368024 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2368024 SequenceAndFeatures</partinfo> | ||
Revision as of 06:06, 4 October 2017
Introduction
His+T1R3 overlap
In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-6*His tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.
Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and His tag. And hope to show the location of T1R3 through immunofluorescence for detecting His tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 12
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 95
- 1000COMPATIBLE WITH RFC[1000]