Difference between revisions of "Part:BBa K2246000:Design"

 
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The blue chromoprotein is used due to is visibility to the naked eye and its small size compared with other reporters.
 
The blue chromoprotein is used due to is visibility to the naked eye and its small size compared with other reporters.
 
The promoter and terminator used work with T7 RNApol due to its high transcription rate.
 
The promoter and terminator used work with T7 RNApol due to its high transcription rate.
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It has a BamHI and a HinCII cut sites around the region where the siRNA should be inserted, those enzymes cut sites were choosen, as they do not hybridize between each other, thus making the perfect form double stranded siRNA formation.  
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It has a BamHI and a HinCII cut sites around the region where the siRNA should be inserted, those enzymes cut sites were choosen, as they do not hybridize between each other, thus making the perfect form double stranded siRNA formation. A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.
  
  

Latest revision as of 02:03, 4 October 2017


BSLA: siRNA production and reporter cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The blue chromoprotein is used due to is visibility to the naked eye and its small size compared with other reporters. The promoter and terminator used work with T7 RNApol due to its high transcription rate. It has a BamHI and a HinCII cut sites around the region where the siRNA should be inserted, those enzymes cut sites were choosen, as they do not hybridize between each other, thus making the perfect form double stranded siRNA formation. A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.


Source

Synthetic part.

References