Difference between revisions of "Part:BBa K2206008:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
We ensured that there are no stop codons between the start codon and the protein coding sequence.  
+
No terminators are needed as the pSB1C3 plasmid contains terminators.
 
+
  
 +
A non-leaky inducible promoter is needed to amplify the constructs in E.coli as quite a high concentration of the transcripts is toxic to E.coli.
  
 
===Source===
 
===Source===

Revision as of 23:04, 2 October 2017


Toehold switch for hsa-miR-15b-5p with luciferase and inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2102


Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.

A non-leaky inducible promoter is needed to amplify the constructs in E.coli as quite a high concentration of the transcripts is toxic to E.coli.

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References