Difference between revisions of "Part:BBa K2206004:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We ensured that there are no stop codons between the start codon and the protein coding sequence. This part contains an RBS.
+
No terminators are needed as the pSB1C3 plasmid contains terminators.
  
  

Revision as of 22:51, 2 October 2017


Toehold switch for hsa-miR-15b-5p with luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 928


Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.


Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT

References