Difference between revisions of "Part:BBa K2206001:Design"
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===Design Notes=== | ===Design Notes=== | ||
We ensured that there are no stop codons between the start codon and the protein coding sequence. | We ensured that there are no stop codons between the start codon and the protein coding sequence. | ||
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The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most. | The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most. | ||
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The distance between the start codon and the protein coding region has to be a multiple of three. | The distance between the start codon and the protein coding region has to be a multiple of three. | ||
Revision as of 21:01, 2 October 2017
Toehold switch for hsa-miR-27b-3p
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ensured that there are no stop codons between the start codon and the protein coding sequence.
The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.
The distance between the start codon and the protein coding region has to be a multiple of three.
For more detail visit: http://2017.igem.org/Team:CLSB-UK
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.