Difference between revisions of "Part:BBa K2206001:Design"

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===Design Notes===
 
===Design Notes===
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We ensured that there are no stop codons between the start codon and the protein coding sequence. This part contains an RBS.
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We ensured that there are no stop codons between the start codon and the protein coding sequence.  
  
  
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The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.
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The distance between the start codon and the protein coding region has to be a multiple of three.
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For more detail visit: http://2017.igem.org/Team:CLSB-UK
  
 
===Source===
 
===Source===

Revision as of 21:00, 2 October 2017


Toehold switch for hsa-miR-27b-3p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that there are no stop codons between the start codon and the protein coding sequence.


The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.


The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References