Difference between revisions of "Part:BBa K2206000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | There must be no stop codons between the start codon and the protein coding sequence. | |
The RBS and start codon should not be paired to anything. | The RBS and start codon should not be paired to anything. | ||
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The distance between the start codon and the protein coding region has to be a multiple of three. | The distance between the start codon and the protein coding region has to be a multiple of three. | ||
| − | For more detail visit: http://2017.igem.org/Team:CLSB-UK | + | For more detail visit: http://2017.igem.org/Team:CLSB-UK |
===Source=== | ===Source=== | ||
Revision as of 20:59, 2 October 2017
Toehold switch for hsa-miR-15b-5p
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There must be no stop codons between the start codon and the protein coding sequence.
The RBS and start codon should not be paired to anything.
The distance between the start codon and the protein coding region has to be a multiple of three.
For more detail visit: http://2017.igem.org/Team:CLSB-UK
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.