Difference between revisions of "Part:BBa K2206000:Design"

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===Design Notes===
 
===Design Notes===
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We ensured that there are no stop codons between the start codon and the protein coding sequence. This part contains an RBS.
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We ensured that there are no stop codons between the start codon and the protein coding sequence.  
 +
 
 +
The RBS and start codon should not be paired to anything.  
 +
 
 +
The distance between the start codon and the protein coding region has to be a multiple of three.
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For more detail visit: http://2017.igem.org/Team:CLSB-UK
  
 
===Source===
 
===Source===

Revision as of 20:49, 2 October 2017


Toehold switch for hsa-miR-15b-5p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ensured that there are no stop codons between the start codon and the protein coding sequence.

The RBS and start codon should not be paired to anything.

The distance between the start codon and the protein coding region has to be a multiple of three.

For more detail visit: http://2017.igem.org/Team:CLSB-UK

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References