Difference between revisions of "Part:BBa K2206000"
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Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region.  
 
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region.  
  
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This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-15b-5p. Therefore, in the presence of hsa-mir-15b-5p, translation of the downstream protein coding region occurs.  
+
This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-15b-5p. In the presence of hsa-mir-15b-5p, translation of the downstream protein coding region occurs.  
  
 
This part can be used to regulate the expression of any protein in response to hsa-mir-15b-5p. This part contains a strong RBS.
 
This part can be used to regulate the expression of any protein in response to hsa-mir-15b-5p. This part contains a strong RBS.

Revision as of 19:00, 2 October 2017


Toehold switch for hsa-miR-15b-5p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-15b-5p. In the presence of hsa-mir-15b-5p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to hsa-mir-15b-5p. This part contains a strong RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]