Difference between revisions of "Part:BBa K2368010"

 
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__NOTOC__
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<h1>Introduction</h1>
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<partinfo>BBa_K2368010 short</partinfo>
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<p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.<p>
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<p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and Myc tag. And hope to show the location of T1R3 through immunofluorescence for detecting Myc tag.<p>
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===Usage and Biology===
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2368010 parameters</partinfo>
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Revision as of 06:12, 2 October 2017


Introduction

myC+T1R3 overlap

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R3-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R3’s expression and location.<p> <p>Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and Myc tag. And hope to show the location of T1R3 through immunofluorescence for detecting Myc tag.<p>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]