Difference between revisions of "Part:BBa K2215004"
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This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader". With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium. (Levin et al., 2017) | This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader". With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium. (Levin et al., 2017) | ||
| − | Because this promoter is pulled from the Symbiodinium genome, it should therefore be utilized in Symbiodinum only. | + | Because this promoter is pulled from the Symbiodinium genome, it should therefore be utilized in Symbiodinum only or optimized for other species. |
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Revision as of 03:57, 2 October 2017
Putative Symbiodinium Promoter
This is putative Symbiodinium promoter that is believed to work best in Symbiodinium due to its unique features, such as a "TTTT-box rather than a TATA box, a unique transcription start site branch point, and acceptor for the dinoflagellate spliced leader". With this promoter, it should allow for more expression and stability of transgenes introduced in Symbiodinium. (Levin et al., 2017) Because this promoter is pulled from the Symbiodinium genome, it should therefore be utilized in Symbiodinum only or optimized for other species.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 6
Illegal suffix found in sequence at 971 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal SpeI site found at 972
Illegal PstI site found at 986
Illegal NotI site found at 12
Illegal NotI site found at 979 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal BglII site found at 360
Illegal BglII site found at 438
Illegal BglII site found at 790
Illegal XhoI site found at 442 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 6
Illegal suffix found in sequence at 972 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 6
Illegal XbaI site found at 21
Illegal SpeI site found at 972
Illegal PstI site found at 986
Illegal AgeI site found at 308 - 1000COMPATIBLE WITH RFC[1000]