Difference between revisions of "Part:BBa K2368013"

 
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<h1>Introduction</h1>
 
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In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein &#945;subunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilGFP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilGFP.
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<h1>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein &#945;subunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilGFP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilGFP.
  
 
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<span class='h3bb'>Sequence and Features</span>
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<h2>Sequence and Features</h2>
 
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Revision as of 03:30, 2 October 2017


Introduction

T1R3-YCP

In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilGFP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilGFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]