Difference between revisions of "Part:BBa K2368012"
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| + | <h1>Introduction</h1> | ||
<partinfo>BBa_K2368012 short</partinfo> | <partinfo>BBa_K2368012 short</partinfo> | ||
| − | In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R2- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and C-terminal of amilGFP. And hope to show the location of T1R2 through fluorescence microscope for detecting amilGFP. | + | <p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R2- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and C-terminal of amilGFP. And hope to show the location of T1R2 through fluorescence microscope for detecting amilGFP.</p> |
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| − | < | + | <h2>Sequence and Features</h2> |
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Revision as of 03:28, 2 October 2017
Introduction
T1R2-YCP
In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R2- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and C-terminal of amilGFP. And hope to show the location of T1R2 through fluorescence microscope for detecting amilGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]