Difference between revisions of "Part:BBa K2423005:Design"

 
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===References===
 
===References===
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1. Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, et al. Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proc Natl Acad Sci USA. 2014 Aug 19;111(33):12246–51.
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2. Ahrazem O, Rubio-Moraga A, Berman J, Capell T, Christou P, Zhu C, et al. The carotenoid cleavage dioxygenase CCD2 catalysing the synthesis of crocetin in spring crocuses and saffron is a plastidial enzyme. New Phytol. 2016 Jan 1;209(2):650–63.
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3. Crocus ancyrensis carotenoid cleavage dioxygenase (CCD2) mRNA, complete cds. 2015 Nov 4; Available from: http://www.ncbi.nlm.nih.gov/nuccore/KP792756.1

Revision as of 19:51, 1 October 2017


CaCCD2 with BBa_J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 837


Design Notes

When designing this part we initially took the coding sequence from KP792756 and codon optimized it for Escherichia Coli using IDT's tool. We repeatedly used it until there were no restriction sites for EcoRI, XbaI, SpeI and PstI. The choice of J04500 came from that we wanted to be able to induce the production of our protein using IPTG.


Source

Originally, the group enzymes was discovered by Frusciante et al. (2014) through deep transcriptome analysis. More specific information about CaCCD2 came approximately one year later Ahrazem et al. (2015). The sequencing they found there has the GenBank acession number KP792756.

References

1. Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, et al. Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proc Natl Acad Sci USA. 2014 Aug 19;111(33):12246–51.

2. Ahrazem O, Rubio-Moraga A, Berman J, Capell T, Christou P, Zhu C, et al. The carotenoid cleavage dioxygenase CCD2 catalysing the synthesis of crocetin in spring crocuses and saffron is a plastidial enzyme. New Phytol. 2016 Jan 1;209(2):650–63.

3. Crocus ancyrensis carotenoid cleavage dioxygenase (CCD2) mRNA, complete cds. 2015 Nov 4; Available from: http://www.ncbi.nlm.nih.gov/nuccore/KP792756.1