Difference between revisions of "Part:BBa K2255003"
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<partinfo>BBa_K2255003 short</partinfo> | <partinfo>BBa_K2255003 short</partinfo> | ||
| − | This tag provides multiple fixation sites: a streptavidine and a histidine tag. Between those tag is a TEV protease | + | This tag provides multiple fixation sites: a streptavidine and a histidine tag. Between those tag is a TEV protease cutting site. |
This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain. | This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain. | ||
Revision as of 16:08, 29 September 2017
Multi-Tag (Rfc 25)
This tag provides multiple fixation sites: a streptavidine and a histidine tag. Between those tag is a TEV protease cutting site.
This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]