Difference between revisions of "Part:BBa K2200006"
| Line 11: | Line 11: | ||
The principle behind this “device” is that when viruses invade bacterias , the self-protection system of bacteria detect the viruses’ DNA. The bacteria produces two kinds pf RNA, one of which contains a sequence that matches the DNA of invading viruses, and this sequence is regarded as guide RNA(also small guide RNA, sgRNA). These two RNAs form a complex with Cas9 protein. When sgRNA finds its target, Cas9( the DNA scissor) then cuts the target DNA sequence. | The principle behind this “device” is that when viruses invade bacterias , the self-protection system of bacteria detect the viruses’ DNA. The bacteria produces two kinds pf RNA, one of which contains a sequence that matches the DNA of invading viruses, and this sequence is regarded as guide RNA(also small guide RNA, sgRNA). These two RNAs form a complex with Cas9 protein. When sgRNA finds its target, Cas9( the DNA scissor) then cuts the target DNA sequence. | ||
In order to conduct this principle, which is called CRISPR-Cas9, we designed a sgRNA that matches our experimental BRAF cell DNA. This sgRNA then brings Cas9 to its target to cut off the mutant DNA. It then causes the death of those cancer cells. Using this method, we are theoretically capable of targeting any tumor cells. This kind of innovated and precise methodology can be adopted to track patients throughout their life without getting their normal cells into danger and within suppressing the dispersion of cancer cells. | In order to conduct this principle, which is called CRISPR-Cas9, we designed a sgRNA that matches our experimental BRAF cell DNA. This sgRNA then brings Cas9 to its target to cut off the mutant DNA. It then causes the death of those cancer cells. Using this method, we are theoretically capable of targeting any tumor cells. This kind of innovated and precise methodology can be adopted to track patients throughout their life without getting their normal cells into danger and within suppressing the dispersion of cancer cells. | ||
| − | U6 promoter drives the expression of sgRNA and HEf1A promoter drives the expression of Cas9.< | + | U6 promoter drives the expression of sgRNA and HEf1A promoter drives the expression of Cas9. |
| + | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2200006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2200006 SequenceAndFeatures</partinfo> | ||
Revision as of 11:27, 28 September 2017
pU6+sgRNA+pHEf1A+hCas9
This part is consisted of Human U6 promoter, sgRNA that targets the specific BRAF sequence. With its high flexibility of being devised to target different tumor gene sequences, it has a great market upon innovating cancer treatments since it will revolutionize this market by personalizing the remedy.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5117
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
Illegal XhoI site found at 1262 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 997
Illegal NgoMIV site found at 4214
Illegal NgoMIV site found at 5123
Illegal AgeI site found at 375 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 5242
Illegal SapI.rc site found at 2633
Illegal SapI.rc site found at 2875