Difference between revisions of "Part:BBa K2255006"
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Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''. | Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''. | ||
| − | This part was design with Freiburg ( | + | This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. |
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Revision as of 16:32, 26 September 2017
EPS-Depolymerase (Rfc25)
Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. Xanthomonas is a close relative of Xylella fastidiosa.
This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]