Difference between revisions of "Part:BBa K2255003"
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This tag provides multiple fixation site: streptavidine and a histidine tag. Between those tag the is a TEV protease clivation site.
 
This tag provides multiple fixation site: streptavidine and a histidine tag. Between those tag the is a TEV protease clivation site.
  
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This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
+
This part was design with Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:19, 26 September 2017


Multi-Tag (Rfc 25)

This tag provides multiple fixation site: streptavidine and a histidine tag. Between those tag the is a TEV protease clivation site.

This part was design with Freiburg (Rfc25) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]