Difference between revisions of "Part:BBa K2294444"

 
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<partinfo>BBa_K2294444 short</partinfo>
 
<partinfo>BBa_K2294444 short</partinfo>
  
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This is a RFC[1000] compatible version of the BBa_E0040 GFP part. The changes are: 1) A silent mutation to remove a BsaI recognition site. 2) RFC[1000] specific prefix and suffix with CDS fusion sites (AATG in the 5' end; GCTT in the 3' end).
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This is a RFC[1000] compatible version of the BBa_E0040 GFP part (https://parts.igem.org/Part:BBa_E0040).  
 +
 
 +
The changes are:  
 +
1) A silent mutation to remove a BsaI recognition site.  
 +
2) RFC[1000] specific prefix and suffix with CDS fusion sites (AATG in the 5' end; GCTT in the 3' end).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:57, 25 September 2017


green fluorescent protein derived from jellyfish Aequeora victoria GFP RFC1000 compatible

This is a RFC[1000] compatible version of the BBa_E0040 GFP part (https://parts.igem.org/Part:BBa_E0040).

The changes are: 1) A silent mutation to remove a BsaI recognition site. 2) RFC[1000] specific prefix and suffix with CDS fusion sites (AATG in the 5' end; GCTT in the 3' end).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]