Difference between revisions of "Part:BBa K2201200"
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Revision as of 20:34, 23 September 2017
Tyrosyl tRNA/aminoacyl-synthetase for the incorporation of 2-nitrophenylalanine
To get the noncanonical amino acid 2-nitrophenylalanine (2-NPA) incorporated into a protein auch as the fusion protein of K2201321, a sequence with a TAG is needed along with an additional tRNA synthetase (RS) that is able to load the ncAA to the tRNA. The ribosome is then able to incorporate this amino acid at an amber codon if the target protein with the amber codon and a plasmid with the 2NPA-RS and tRNA are co-transformed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1153
Illegal BamHI site found at 1159 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 404
Illegal NgoMIV site found at 1185
Illegal NgoMIV site found at 1645 - 1000COMPATIBLE WITH RFC[1000]