Difference between revisions of "Part:BBa K2273066"
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Revision as of 23:21, 22 September 2017
SpoIID signal peptide of B. subtilis lytic transglycosylase
The signal peptide (amino acids 1 to 34) of Bacillus subtilis lytic transglycosylase (cell wall hydrolase involved in dissolution of the septal cell wall, Uniprot [http://www.uniprot.org/uniprot/P07372 P07372]) targets for protein secretion via the Sec-SRP secretion pathway (Brockmeier et al, 2006).
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
| Prefix with | EcoRI, NotI, XbaI and SD | GAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAA |
| Suffix with | AgeI, SpeI, NotI and PstI | ACCGGTTAATACTAGTAGCGGCCGCTGCAGA |
Sites of restriction enzymes generating compatible overhangs are indicated by sharing one color. (EcoRI and PstI are marked in blue, NotI in green, XbaI and SpeI in red and AgeI in orange. Additionally, the Shine-Dalgarno sequence is marked in silver and the stop codon is underlined.)
This part is used in the 2017 TU Dresden iGEM project [http://2017.igem.org/Team:TU_Dresden EncaBcillus - It's a trap!] and part of the Signal Peptide Toolbox.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]