Difference between revisions of "Part:BBa K2273066:Design"

 
(→‎Source)
Line 11: Line 11:
  
  
−
===Source===
+
The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome using the primers listed below.
−
 
+
<br>
−
The SpoIID signal peptide of <i>B. subtilis</i> lytic transglycosylase was amplified via PCR from the <i>B. subtilis</i> wild type W168 genome.
+
<table>
 +
<tr>
 +
<td width="150">SpoIID_SP fwd</td>
 +
<td>gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT</td>
 +
</tr>
 +
<tr>
 +
<td>SpoIID_SP rev</td>
 +
<td>gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT</td>
 +
</tr>
 +
</table>
 +
Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.
  
 
===References===
 
===References===

Revision as of 18:51, 22 September 2017


SpoIID signal peptide of B. subtilis lytic transglycosylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tba


The SpoIID signal peptide of B. subtilis lytic transglycosylase was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.

SpoIID_SP fwd gatcGAATTCGCGGCCGCTTCTAGATAAGGAGGTCAAAAATGAAACAATTCGCAATCACACTAT
SpoIID_SP rev gatcTCTGCAGCGGCCGCTACTAGTATTAACCGGTGGCCCCCGCTTCCTTATT

Following amplification, the signal peptide was digested using EcoRI and PstI and ligated into pSB1C3.

References