Difference between revisions of "Part:BBa J100328:Experience"
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| − | + | Cell type used was JM109. Data is average red fluorescence of 3 identical samples of each clone. Cells were grown in LB + AMP media. When ATC was present, its concentration was 0.2 micrograms/mL. | |
| + | X1-3 were all believed to be J100328 (our designed promoter) in J100205 (repClone). Each was isolated based on perceived level of red fluorescence (X1 being the most fluorescent and X3 being the least). | ||
| + | WT was R0040 (wild type tet promoter). | ||
| + | Negative control was J100205 alone. | ||
| + | Positive control was J04450 (a sequence known to have a strong promoter, RBS, and RFP). | ||
Latest revision as of 14:20, 21 September 2017
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Cell type used was JM109. Data is average red fluorescence of 3 identical samples of each clone. Cells were grown in LB + AMP media. When ATC was present, its concentration was 0.2 micrograms/mL. X1-3 were all believed to be J100328 (our designed promoter) in J100205 (repClone). Each was isolated based on perceived level of red fluorescence (X1 being the most fluorescent and X3 being the least). WT was R0040 (wild type tet promoter). Negative control was J100205 alone. Positive control was J04450 (a sequence known to have a strong promoter, RBS, and RFP).
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