Difference between revisions of "Part:BBa K2269004"
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<partinfo>BBa_K2269004 short</partinfo>
 
<partinfo>BBa_K2269004 short</partinfo>
  
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Chimeric promoter containing Etr8 (an E-responsive operator motif) fused to the minimal CMV promoter (Müller et al., 2014)
+
Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:37, 20 September 2017


Register Assembly 1/3 (Luc)

Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]