Difference between revisions of "Part:BBa K2269003"

 
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<partinfo>BBa_K2269003 short</partinfo>
 
<partinfo>BBa_K2269003 short</partinfo>
  
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E-responsive operator motif
+
Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.
  
 
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Revision as of 17:37, 18 September 2017


Register Assembly 3/3 (FT)

Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 361
    Illegal XbaI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 361
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 361
    Illegal BglII site found at 84
    Illegal BglII site found at 191
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 361
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 361
    Illegal XbaI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]