Difference between revisions of "Part:BBa K2255018"
| Line 4: | Line 4: | ||
This part is sequence coding for the D1 and D2 of the protein 3 of XacF1, a filamentous phage that target X.fuscans. Those domains are required for phage adsorption and entry. | This part is sequence coding for the D1 and D2 of the protein 3 of XacF1, a filamentous phage that target X.fuscans. Those domains are required for phage adsorption and entry. | ||
| + | |||
| + | This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:19, 30 August 2017
p3_X.fuscans (Rfc25)
This part is sequence coding for the D1 and D2 of the protein 3 of XacF1, a filamentous phage that target X.fuscans. Those domains are required for phage adsorption and entry.
This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 223
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]