Difference between revisions of "Part:BBa K2255006"
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Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''.
 
Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. ''Xanthomonas'' is a close relative of ''Xylella fastidiosa''.
  
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This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
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This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site  NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:15, 30 August 2017


EPS-Depolymerase (Rfc25)

Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids, from the phage vB_XveM_DIBBI which contain two function one structural and one pectin lyase. Xanthomonas is a close relative of Xylella fastidiosa.

This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]