Difference between revisions of "Part:BBa K2255010"
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This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P.aeruginosa. Those domains are required for phage adsorption and entry.
 
This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P.aeruginosa. Those domains are required for phage adsorption and entry.
  
−
This part was design with Freiburg (RFC[25]) as extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
+
This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:07, 30 August 2017


p3_P.aeruginosa (Rfc25)

This part is sequence coding for the D1 and D2 of the protein 3 of Pf3, a filamentous phage that target P.aeruginosa. Those domains are required for phage adsorption and entry.

This part was design with Freiburg (RFC[25]) extension. Thus, it contain the restriction site NgoMIV and AgeI that are compatible and allow the missing of a start and stop codon, which ease the assemble of multiple protein domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 814