Difference between revisions of "Part:BBa I732096"

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<partinfo>BBa_I732096 short</partinfo>
 
<partinfo>BBa_I732096 short</partinfo>
  
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
 
   
 
   
 
* It's named as DPv2C as on pSB1A3 plasmid.
 
* It's named as DPv2C as on pSB1A3 plasmid.

Latest revision as of 14:40, 14 October 2007

Promoter Activity Reporter (LacZ-alpha and GFP)

Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

  • It's named as DPv2C as on pSB1A3 plasmid.
  • It's named as DPv2F as on pSB3K5 plasmid.
  • It's named as DPv2N as on pSB3C5 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 949