Difference between revisions of "Part:BBa I732096"

Revision as of 14:39, 14 October 2007

Promoter Activity Reporter (LacZ-alpha and GFP)

Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

It's named as DPv2C as on pSB1A3 plasmid.
It's named as DPv2F as on pSB3K5 plasmid.
It's named as DPv2N as on pSB3C5 plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3
Illegal BamHI site found at 12
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 949

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 __NOTOC__
 <partinfo>BBa_I732096 short</partinfo>
+Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
-A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB1A3.
+* It's named as DPv2C as on pSB1A3 plasmid.
-For more information please refer to BBa_I732401.
+* It's named as DPv2F as on pSB3K5 plasmid.
+* It's named as DPv2N as on pSB3C5 plasmid.
 <!-- Add more about the biology of this part here