Difference between revisions of "Part:BBa I732095"

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<partinfo>BBa_I732095 short</partinfo>
 
<partinfo>BBa_I732095 short</partinfo>
  
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A Double Reporter (LacZ-alpha and RFP) loaded on pSB1A3. It's named as Double Reporter version2-B.
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and RFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
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The double reporter LacZ-alpha and RFP loaded on pSB3K5 is called Double Reporter version2-E.
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* It's named as DPv2B as on pSB1A3 plasmid.
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* It's named as DPv2E as on pSB3K5 plasmid.
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The double reporter LacZ-alpha and RFP loaded on pSB3C5 is called Double Reporter version2-M.  
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* It's named as DPv2M as on pSB3C5 plasmid.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 14:38, 14 October 2007

Promoter Activity Reporter (LacZ-alpha and RFP)

Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and RFP. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

  • It's named as DPv2B as on pSB1A3 plasmid.
  • It's named as DPv2E as on pSB3K5 plasmid.
  • It's named as DPv2M as on pSB3C5 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 860
    Illegal AgeI site found at 972
  • 1000
    COMPATIBLE WITH RFC[1000]