Difference between revisions of "Part:BBa I732094"
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<partinfo>BBa_I732094 short</partinfo>
 
<partinfo>BBa_I732094 short</partinfo>
  
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A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB1A3. It's named as Double Reporter version2-A.
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Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
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The double reporter LacZ-alpha and GFP-AAV loaded on pSB3K5 is called Double Reporter version2-D.
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It's named as DPv2A as on pSB1A3 plasmid.
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It's named as DPv2D as on pSB3K5 plasmid.
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The double reporter LacZ-alpha and GFP-AAV loaded on pSB3C5 is called Double Reporter version2-L.
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It's named as DPv2L as on pSB3C5 plasmid.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:37, 14 October 2007

Promoter Activity Reporter (LacZ-alpha and GFP-AAV)

Promoter can be inserted into the adapter containing BglII and BamHI sites. There are two downstream reporters, LacZ-alpha and GFP-AAV. The activity of inserted promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

It's named as DPv2A as on pSB1A3 plasmid. It's named as DPv2D as on pSB3K5 plasmid. It's named as DPv2L as on pSB3C5 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 949