Difference between revisions of "Part:BBa K2333004"

 
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__NOTOC__
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<partinfo>BBa_K2333004 short</partinfo>
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This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon). <b>LINK TO mf-Lon goes here </b>The Lon protease efficiently recognize and degrade C-terminal ssrA-tagged proteins, functioning in a similar way as ClpXP protease in most gram-negative bacteria. This Lon protease system is orthogonal to E. coli’s endogenous protein degradation machinery. This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). pdt#3 is used as the parental tag to create #3a-#3e by changing the pdt residues 13–15 for mutagenesis because this region is essential for Lon-mediated degradation. Of this series of tags, this tag is relatively weak, and has a slow degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates.
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Note: pdt #3a varies from pdt #3 in that residues 13-15 change from “PTF” to “AQP”.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2333004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2333004 parameters</partinfo>
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Revision as of 22:48, 14 June 2017


mf-Lon Protein Degradation Tag D (medium)

This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon). LINK TO mf-Lon goes here The Lon protease efficiently recognize and degrade C-terminal ssrA-tagged proteins, functioning in a similar way as ClpXP protease in most gram-negative bacteria. This Lon protease system is orthogonal to E. coli’s endogenous protein degradation machinery. This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). pdt#3 is used as the parental tag to create #3a-#3e by changing the pdt residues 13–15 for mutagenesis because this region is essential for Lon-mediated degradation. Of this series of tags, this tag is relatively weak, and has a slow degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates. Note: pdt #3a varies from pdt #3 in that residues 13-15 change from “PTF” to “AQP”.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]