Difference between revisions of "Part:BBa M50059"
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This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically. | This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically. | ||
Therefore, bacteria transformed with this sequence will look brown/black. | Therefore, bacteria transformed with this sequence will look brown/black. | ||
| + | The entire sequence was optimized for Escherichia coli. | ||
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Latest revision as of 06:27, 11 June 2017
Blackteria: Genetic sequence for the production of melanin
The MelA reporter (BBa_M50057) with a strong RBS added to it. A T5 promoter and a PhageT7 terminator have been added to it as well. This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically. Therefore, bacteria transformed with this sequence will look brown/black. The entire sequence was optimized for Escherichia coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 239
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 614