Difference between revisions of "Part:BBa M50059:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
A T-5 promoter is easily inducible and therefore an effective promoter to test the melanin sequence. The strong RBS makes sure that the ribosome binds. The double terminating sequence makes sure transcription terminates.
 
A T-5 promoter is easily inducible and therefore an effective promoter to test the melanin sequence. The strong RBS makes sure that the ribosome binds. The double terminating sequence makes sure transcription terminates.
 +
The original DNA sequence comes from part Bba_K274001 which obtained sequence from Rhizobium etli CFN42. However, we optimized the sequence for E. coli so that it will work better.
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
Rhizobium etli CFN42
+
Rhizobium etli CFN42, unaltered gene sequence obtained from: Bba_K274001
  
 
===References===
 
===References===
 +
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2006.03013.x/full , make-up of the media including necessary Cu and L-tyrosine precursor.
 +
http://2009.igem.org/Team:Tokyo_Tech/BlackenedEcoli#Materials_and_Methods

Revision as of 00:00, 27 April 2017


Blackteria: Genetic sequence for the production of melanin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 614


Design Notes

A T-5 promoter is easily inducible and therefore an effective promoter to test the melanin sequence. The strong RBS makes sure that the ribosome binds. The double terminating sequence makes sure transcription terminates. The original DNA sequence comes from part Bba_K274001 which obtained sequence from Rhizobium etli CFN42. However, we optimized the sequence for E. coli so that it will work better.


Source

Rhizobium etli CFN42, unaltered gene sequence obtained from: Bba_K274001

References

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2006.03013.x/full , make-up of the media including necessary Cu and L-tyrosine precursor. http://2009.igem.org/Team:Tokyo_Tech/BlackenedEcoli#Materials_and_Methods