Difference between revisions of "Part:BBa M50059"

 
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<partinfo>BBa_M50059 short</partinfo>
 
<partinfo>BBa_M50059 short</partinfo>
  
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BBa_M50057 part with a strong RBS added to it. A T-5 promoter and a PhageT7 terminator have been added to it as well.  
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The MelA reporter (BBa_M50057) with a strong RBS added to it. A T-5 promoter and a PhageT7 terminator have been added to it as well.  
 
This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically.
 
This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically.
 +
Therefore, bacteria transformed with this sequence will look brown/black.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:56, 26 April 2017


Blackteria: Genetic sequence for the production of melanin

The MelA reporter (BBa_M50057) with a strong RBS added to it. A T-5 promoter and a PhageT7 terminator have been added to it as well. This sequence will enable bacteria, under the presence of Copper, to transform l-tyrosine to dopaquinone which turns into melanin nonenzymatically. Therefore, bacteria transformed with this sequence will look brown/black.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 614