Difference between revisions of "Part:BBa J100309"

 
 
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actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein.  
 
actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein.  
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Latest revision as of 16:29, 16 February 2017


actClone Red with wt full OmpR promoter

actClone Red is a construct we designed to allow students and researchers to investigate the OmpR promoter. The RFP reporter system in actClone provides phenotypic evidence of the action of the OmpR promoter for the transcription of RFP. This construct uses the wild type OmpR promoter sequence. Researchers can manipulate this sequence by mutating, inserting, or deleting bases. They can then compare RFP expression in their new constructs to RFP expression using the wild-type promoter sequence (R0082). This will allow researchers and biology students to explore sequence requirements for the interactions between the OmpR promoter and phosphorylated OmpR regulatory protein.

J100309.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1557
    Illegal AgeI site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 702