Difference between revisions of "Part:BBa M50027:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required. | + | There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1). |
===Source=== | ===Source=== | ||
Latest revision as of 07:50, 12 December 2016
LuxS Synthase for Autoinducer-2 Pathway in E. coli
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 50
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 50
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 155
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 50
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 50
Illegal AgeI site found at 253 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There were no modifications of the original genomic sequence. When using the codon optimization tool from Gene Designer 2.0, there were no alterations required. Our inspiration for this project stemmed from reading the literature about possible sources for possible endogenous pathways for the control of bacterial growth. Some papers described quorum sensing pathways, specifically Autoinducer-2, as having metabolic and growth control properties (See reference 1).
Source
The sequence was sourced from Uniprot under the name P45578
References
1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.