Difference between revisions of "Part:BBa M50028:Design"

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===Design Notes===
 
===Design Notes===
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We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analysis the original sequence and there was no indication of codon optimization, etc.
+
We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analyze the original sequence and there was no indication of codon optimization, etc.
  
 
===Source===
 
===Source===

Revision as of 07:39, 12 December 2016


LsrR Repressor Protein for the Autoinducer-2 Pathway in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did not have to make any alterations of the original DNA sequence. We used Gene Designer 2.0 to analyze the original sequence and there was no indication of codon optimization, etc.

Source

The genetic sequence was sourced from Uniprot under the name P76141 (LSRR_ECOLI).

References

1. Xavier KB and Bassler BL. 2005. Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli. J Bacteriol. 187(1): 238-248.