Difference between revisions of "Part:BBa M50046:Design"
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===Source=== | ===Source=== | ||
Latest revision as of 07:27, 12 December 2016
SV40 polyA Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 36
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 16
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 36
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 36
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This poly-adenosine terminator will stop transcription for mammalian cells.
Source
TA Robert Chen's plasmid