Difference between revisions of "Part:BBa M50055:Design"

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===Design Notes===
 
===Design Notes===
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We added an eGFP sequence downstream of our atrazine riboswitch so the the riboswitch would control translation of the eGFP.
 
We added an eGFP sequence downstream of our atrazine riboswitch so the the riboswitch would control translation of the eGFP.
  

Revision as of 06:49, 12 December 2016


Atrazine biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 760


Design Notes

We added an eGFP sequence downstream of our atrazine riboswitch so the the riboswitch would control translation of the eGFP.

Source

Riboswitch components: BBa_J89000

Atrazine aptamer: In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine, IJMS International Journal of Molecular Sciences

eGFP sequence: BBa_K598009

References

https://parts.igem.org/Part:BBa_K598009

https://parts.igem.org/Part:BBa_J89000

Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332