Difference between revisions of "Part:BBa M50011:Design"
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===Source=== | ===Source=== | ||
| − | This genetic sequence comes from the work of Hydrogen Peroxide Sensor HyPer. | + | This genetic sequence comes from the work of Hydrogen Peroxide Sensor HyPer. The research into the HyPer gene was created by Belousov et al. |
===References=== | ===References=== | ||
Revision as of 03:34, 12 December 2016
This is an E. coli specific sensor for hydrogen peroxide.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 844
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25
Illegal SapI.rc site found at 709
Design Notes
This device is designed as a rhamnose - inducible biological sensor that allows E. coli to detect the presence of hydrogen peroxide. This sensor consists of a strong RBS, ampicillin resistance, a high copy number at the origin of replication, the gene of interest, the HyPer plasmid, and a transcription terminator from DNA 2.0. Specifically, a strong RBS was selected in order to ensure robust expression of the HyPer gene. Additionally, the plasmid is constructed of DNA 2.0’s default terminator, pT-T7, which is derived from the T7 bacteriophage.
This device is based off of the HyPer gene, which allows cells to sense hydrogen peroxide; however HyPer originally formatted the genetic sequence for mammalian cells, so we converted it to E. coli cells. With regards to the design of the HyPer gene, the Belousov et al. researchers used the knowledge that the E. coli regulatory region, OxyR-RD, is especially sensitive to the presence of hydrogen peroxide. The researchers also inserted a cpYFP into the sensor to demonstrate when hydrogen peroxide is detected.
Source
This genetic sequence comes from the work of Hydrogen Peroxide Sensor HyPer. The research into the HyPer gene was created by Belousov et al.