Difference between revisions of "Part:BBa M50053:Design"
(→Design Notes) |
(→Design Notes) |
||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | We engineered E. coli to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct | + | We engineered <i>E. coli</i> to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct will undergo a conformational change upon binding to glucose such that the cometGFP portion in an excited state will transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair reveals the extent to which FRET and thus glucose binding that has occurred. |
===Source=== | ===Source=== |
Revision as of 02:54, 12 December 2016
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77
Design Notes
We engineered E. coli to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct will undergo a conformational change upon binding to glucose such that the cometGFP portion in an excited state will transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair reveals the extent to which FRET and thus glucose binding that has occurred.
Source
source