Difference between revisions of "Part:BBa M50053:Design"

 
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===Design Notes===
 
===Design Notes===
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design notes
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We engineered E. coli to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct would undergo a conformational change upon binding to glucose such that the cometGFP portion in an excited state would transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair would reveal the extent to which FRET and thus glucose binding that had occurred.
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===Source===
 
===Source===

Revision as of 02:53, 12 December 2016


FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 77


Design Notes

We engineered E. coli to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct would undergo a conformational change upon binding to glucose such that the cometGFP portion in an excited state would transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair would reveal the extent to which FRET and thus glucose binding that had occurred.

Source

source

References