Difference between revisions of "Part:BBa M50021:Experience"

Revision as of 00:42, 12 December 2016

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Applications of BBa_M50021

User Reviews

This part was inserted into an expression vector plasmid by DNA 2.0 containing GFP, so we wanted to develop a heat-response curve quantifying GFP output. After transforming this plasmid into chemically competent E. coli, we selected colonies based on ampicillin resistance and produced 3 liquid cultures of biological replicates. 3 wells each of these cultures (for 9 total samples) were held in a spectrophotometer/plate reader while shaking for 4 hours, with the machine taking readings of CometGFP fluorescence and cell density (OD600) every 10 minutes). After normalizing fluorescence to cell density, we established the following curves at 25ºC, 37ºC, and 42ºC:

UNIQd9f4a3d20d71dde2-partinfo-00000000-QINU UNIQd9f4a3d20d71dde2-partinfo-00000001-QINU

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 After transforming this plasmid into chemically competent E. coli, we selected colonies based on ampicillin resistance and produced 3 liquid cultures of biological replicates.
 wells each of these cultures (for 9 total samples) were held in a spectrophotometer/plate reader while shaking for 4 hours, with the machine taking readings of CometGFP fluorescence and cell density (OD600) every 10 minutes).  After normalizing fluorescence to cell density, we established the following curves at 25ºC, 37ºC, and 42ºC:
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