Difference between revisions of "Part:BBa M50021:Experience"
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| + | This part was inserted into an expression vector plasmid by DNA 2.0 containing GFP, so we wanted to develop a heat-response curve quantifying GFP output. | ||
| + | After transforming this plasmid into chemically competent E. coli, we selected colonies based on ampicillin resistance and produced 3 liquid cultures of biological replicates. | ||
| + | 3 wells each of these cultures (for 9 total samples) were held in a spectrophotometer/plate reader while shaking for 4 hours, with the machine taking readings of CometGFP fluorescence and cell density (OD600) every 10 minutes). After normalizing fluorescence to cell density, we established the following curves at 25ºC, 37ºC, and 42ºC: | ||
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Revision as of 00:40, 12 December 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M50021
User Reviews
UNIQ52bc08744da6ecfb-partinfo-00000000-QINU UNIQ52bc08744da6ecfb-partinfo-00000001-QINU