Difference between revisions of "Part:BBa M50009"

Revision as of 22:17, 11 December 2016

mRuby3 fluorescent protein

mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Unknown
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 16

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 <partinfo>BBa_M50009 short</partinfo>
-mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in FRET analysis as an acceptor fluorophore when paired with a GFP such as cometGFP.
+mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in fluorescent resonance energy transfer (FRET) analysis as an acceptor fluorophore when paired with another compatible fluorescent protein, like a GFP. Emission and absorption spectra must be analyzed for each fluorophore member in the FRET pair to ensure proper overlap and optimal FRET efficiency.
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