Difference between revisions of "Part:BBa M50050:Design"

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===Design Notes===
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==Design==
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=== Special Notes===
 
Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action.
 
Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action.
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===Plasmid Map===
  
 
===Source===
 
===Source===

Revision as of 21:13, 11 December 2016


IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 696
    Illegal PstI site found at 202
    Illegal AgeI site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Special Notes

Designing this part required no modifications. During our design process in DNA 2.0, we ran the sequence through codon optimization and tested the restriction sites, but there were no recommendations that required action.


Plasmid Map

Source

The source of the LuxS gene came from UniProt. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH.

References