Difference between revisions of "Part:BBa M50039"
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This sequence contains a shortened version of the cusR-inducible promoter, a strong RBS, and the gene for cusR. | This sequence contains a shortened version of the cusR-inducible promoter, a strong RBS, and the gene for cusR. | ||
| − | <!-- | + | <!-- CusR is a Cu-sensing regulator, it regulates genes that are related to copper efflux systems under copper stress in aerobic growth, as well as anaerobic growth. It belongs to the two component CusR/CusS system, which responds to an increase in copper concentration. The membrane protein cusS and cytoplasmic protein cusR are always present at low levels within the cell. The presence of copper phosphorylates the membrane protein cusS, which then trans-phosphorylates cytoplasmic cusR. Once cusR is activated, it binds to the appropriate sequence within the cusR-inducible promoter region, initiating bidirectional transcription of cusS and cusR. This pathway increases cusS and cusR concentration in the presence of copper, acting as a positive feedback loop that allows the cell to display an amplified response to toxic copper ions. These proteins then go on to activate downstream proteins that bind the copper ions to inactivate them, thereby increasing the cell’s survivability in copper ion solutions. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:22, 11 December 2016
E. coli positive feedback cusR producer (short promoter)
This sequence contains a shortened version of the cusR-inducible promoter, a strong RBS, and the gene for cusR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 53
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 53
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 53
Illegal AgeI site found at 243 - 1000COMPATIBLE WITH RFC[1000]