Difference between revisions of "Part:BBa M50051"

Revision as of 17:14, 11 December 2016

IPTG Inducible Plasmid for the Expression of LsrR Repressor

This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG.

Usage and Biology

LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 1134
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 1134
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 1134
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 1134
1000
COMPATIBLE WITH RFC[1000]

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 This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species.